High tissue glucose alters intersomitic blood vessels in. As one of three akt isoforms, akt2 plays a key role in the regulation of widely divergent cellular processes in mammals. In this study, zebrafish were used as a model organism to analyze early vascular effects and. Hardcopies of the 4th edition of the zebrafish book can be obtained for a nominal fee from the zebrafish international resource center, 5274 university of oregon, eugene, or 97403 usa. Akt2null zebrafish display impaired glucose homeostasis. Please acknowledge the zebrafish international resource center in all publications resulting from the materials andor services we are providing to you. Please use this form to recommend updates to the information in zfin. To overcome these limitations, circulating tumor cells ctcs represent a. Biomolecules free fulltext preclinical evaluation of the. As one of three akt isoforms, akt2 plays a key role in the regulation of widely divergent cellular. The cells were labeled with abfinity akt recombinant rabbit monoclonal antibody cat.
Hhuman mmouse rrat hmhamster mkmonkey mimink cchicken xxenopus z zebra fish bbovine allall species expected species enclosed in parentheses are predicted to react based on 100% sequence homology. Rat, rabbit, chicken, hamster, cat, dog, human, pig, xenopus laevis, fish, monkey, zebrafish, baboon, african green monkey predicted. Rip2 is an adaptor protein which is essential for the activation of nf. Select appropriate fish antibodies for your research by isotype, epitope, applications and species reactivity. View detailed zebrafish protein antibody specifications by linking to the specific product blocks. The zebrafish book a guide for the laboratory use of zebrafish danio brachydanio rerio by monte westerfield, institute of neuroscience, university of oregon older literature referred to zebrafish as brachydanio rerio. Elevated glucose concentrations lead to increased formation of the highly reactive dicarbonyl methylglyoxal mg, yet the early consequences of mg for development of vascular complications in vivo are poorly understood.
Hyperglycemia causes micro and macrovascular complications in diabetic patients. Nod1 deficiency impairs cd44alck as well as pi3kakt pathway. This is where zebrafish researchers can help each other by sharing antibody information new antibodies, protocols, tips anything that might be helpful. The antiakt and antiphosphoakt antibodies ser473 and the.
Blots were incubated with ir and phosphoakt antibodies, and parallel. Recent advances in akt signaling have focused on understanding cellular processes and identifying. View detailed fish antibody specifications by linking to the specific product blocks. The tcf7l2 gene, encoding the tcf7l2 protein, is located on chromosome 10q25. Zhiyuan gong and his colleagues at the national university of singapore were working with a gene that encodes the green fluorescent protein gfp, originally extracted from a jellyfish, that naturally produced. Immunoassays for akt signaling there are a variety of immunoassays that can assist in monitoring akt antibody levels, many of which are offered by rockland. After confirming the specificity of zebrafish nod1 antibody, we. Zebrafish akt2 is essential for survival, growth, bone development. Biomolecules free fulltext preclinical evaluation of. A genetically modified organism gmo is any organism whose genetic material has been altered using genetic engineering techniques. The central importance of the general phenomenon of sensing of external stimuli by cells has been appreciated for a long time, but has truly become a dominant part of cell and molecular biology research in the past two decades, in part because a description of the dynamic responses of cells to external stimuli is in essence a description of the life process. Confocal immunofluorescent images of c2c12 cells showing nuclear and cytoplasmic localization with akt antibody left, red. The akt antibody was generated using akt, rac, hihghh, vakt2, vakt murine thymoma viral oncogene homolog 1, akt3, akt1, and akt2 as the antigen. Expression of the zebrafish smoa1egfp itself did not lead to tumor.
Akt1, akt2 and akt3 are three closely related isoforms of akt. A novel natural product inspired scaffold with robust. Wholebrain activity mapping onto a zebrafish brain atlas. The tcf7l2 gene contains 619 amino acids and its molecular mass is 67919 da. Oct 30, 2019 welcome to the zfin community antibody wiki. I am working on the enteric nervous system of zebrafish and i am looking for a cellcycle marker to label proliferating cells. Zebrafish antibodies primary antibodies sigmaaldrich. The gene contains 19 exons and has autosomal dominant inheritance.
The original zebrafish or zebra danio, danio rerio is a native of rivers in india and bangladesh. Antibody produced by immunizing rabbits with a synthetic peptide derived from the carboxyterminal sequence of mouse akt. Listed below are antifish antibodies from multiple suppliers. Akt is one of the most actively studied kinase pathways in the basic research and drug development arenas, as it plays an integral role in mediating signals for cell growth, survival, cellcycle progression, differentiation, transcription, translation, and glucose metabolism. If you dont see an antibody in the list below, doublecheck by searching for. Following fixation in 4% pfa, larvae were processed for immunofluorescence study with mouse antibrdu antibody 1. Using genomewide transcriptional profiling and wholemount expression analyses of zebrafish larvae, we have identified hyaluronan synthase 3 has3 as an upregulated gene during caudal fin regeneration. Akt antibodies molecular mechanisms of cancer sigma. Transcription factor 7like 2 tcell specific, hmgbox, also known as tcf7l2 or tcf4, is a protein acting as a transcription factor that, in humans, is encoded by the tcf7l2 gene. Antibodies, primary antibodies, zfish zebrafish, alphabetical listing of zebrafish antibodies.
Akt antibody confirms silencing of akt expression, while the p42 map kinase erk2 antibody is used to control for loading and specificity of akt sirna. Exclusively from the antibody resource, you can buy this superstarter kit, which is a collection of trial sizes of these superstar akt antibodies highly rated and independently tested by us, to test using the specific experimental conditions which your research dictates, so that you can identify which will be the most appropriate for your research project. The monoclonal antibodies were generated with balbc mice against adult zebrafish protein, and clones were selected based on the specific staining patterns. Total akt, used as a loading control, was detected with primary rabbit anti akt antibody cell signaling technology diluted 1. Citations 3822 flow cytometric analysis of lncap cells, untreated green or ly294002treated blue, using phosphoakt ser473 antibody compared to a nonspecific negative control antibody red. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog. Citations 3822 flow cytometric analysis of lncap cells, untreated green or ly294002treated blue, using phospho akt ser473 antibody compared to a nonspecific negative control antibody red.
Western blot analysis of extracts from nih3t3 cells, untreated or treated with pdgf, wortmannin, ly294002. Akt, also referred to as protein kinase b, is a serinethreonine kinase found as part of the insulin, insulinlike growth factor1 igf1 4 phosphatidylinositol 3kinase pi3kphosphatidylinositoldependent kinase1 pdk1 pathway. Traditionally, studies to address the characterization of mechanisms promoting tumor aggressiveness and progression have been focused only on primary tumor analyses, which could provide relevant information but have limitations to really characterize the more aggressive tumor population. Data from 17 fish imaged in the vicinity of the pretectum and anterior hindbrain between 2 and 39 imaging planes per fish, at 3um spacing were registered to the zbrain.
The akt antibody was generated using akt, rac, hihghh, vakt2, v akt murine thymoma viral oncogene homolog 1, akt3, akt1, and akt2 as the antigen. B the 3d larvae labeled with antiacetylated tubulin antibody brown, lateral view with anterior to the left. Online help keyboard shortcuts feed builder whats new. Select fish antibodies from monoclonal antibodies listed below. Total akt, used as a loading control, was detected with primary rabbit antiakt antibody cell signaling technology diluted 1. There are 387 monoclonal zebrafish antibody products, 5433 polyclonal zebrafish antibody products, 37 recombinant zebrafish antibody products. The cellular localization is predicted to be cytoplasmic. Dako products we help pathologists and clinicians make accurate diagnoses that help determine the most effective treatment for cancer patients we are proud to be a renowned provider of complete pathology solutions and flow cytometry reagents, trusted by clinical laboratories around the world in the fight against cancer. It measures three centimeters long and has gold and dark blue stripes. A secondary goat antirabbit antibody conjugated to horseradish peroxidase thermo scientific, rockford, il, usa diluted 1. Due to their striking genomic similarity to humans, regenerative abilities, and shortened life cycle, zebrafish have become an ideal tool for studying developmental processes, gene expression, and underlying mechanisms of cancer and other diseases. Frontiers rip2 is a critical regulator for nlrs signaling. We appreciate as much detail as possible and references as appropriate. Akt antibody from cell signaling technology cell signaling technologys akt antibody is a rabbit polyclonal antibody.
Immunohistochemistry of wholemount and sectioned zebrafish tissue. Upon activation, akt phosphorylates a broad range of substrates involved in metabolism, transcription, translation. Tcf7l2s secondary structure is a helixturnhelix structure. Select appropriate zebrafish protein antibodies for your research by isotype, epitope, applications and species reactivity. Oct 09, 2009 akt, also referred to as protein kinase b, is a serinethreonine kinase found as part of the insulin, insulinlike growth factor1 igf1 4 phosphatidylinositol 3kinase pi3kphosphatidylinositoldependent kinase1 pdk1 pathway. Western blot analysis of extracts from nih3t3 cells, untreated or treated with pdgf, wortmannin, ly294002, rapamycin or pd98059, using phosphoakt ser473 antibody. It plays an important role in mammalian cellular signaling. Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.
Neuroprotective role of the pi3 kinaseakt signaling pathway in. Fgfsignalingdependent sox9a and atoh1a regulate otic neural. Is the zebrafish research community using a subset of specialist antibodies raised for zebrafis. To elucidate the function of akt2 in zebrafish, we generated zebrafish lacking akt2 gene via crisprcas9 technology. The marketplace for antibodies, elisa kits, and other research reagents. Ligands including growth factors, cytokines, hormones and mitogens activate akt signaling via pi3k phosphatidylinositol 3kinase. Caenorhabditis elegans, drosophila melanogaster, ferret, indian muntjac, schizosaccharomyces pombe, zebrafish, silk worm, dictyostelium discoideum, rainbow trout. Aktpkb cell signaling pathwaynovex antibodies, elisas. About these antibodies all antibodies listed on this page were generated by researchers at the institute of neuroscience, university of oregon liu et al. Akt1 is involved in cellular survival pathways by inhibiting apoptotic. Akt2null zebrafish exhibit partial lethality and severe growth deficiency. Background akt, also called protein kinase b pkb, is a serinethreoninespecific protein kinase originally identified as the oncogene in the transforming retrovirus, akt8.
Calcium deficiencyinduced and trp channelregulated. As the leading manufacturer of zebrafish antibodies, genetex offers an extensive portfolio of reagents validated for use in this model system. This is a summary of zebrafish antibody products in labome database. Several distinct zebrafish akt proteins have been identified.
The secondary antibody normally is conjugated to an enzyme such as horseradish peroxidase hrp or alkaline phosphatase ap. Thus, this provides a useful and novel system to understand the. However, its role and underlying mechanisms in zebrafish remain largely unknown. Western blot analysis of extracts from nih3t3 cells, untreated or treated with pdgf for the indicated times, using. A guide for the laboratory use of zebrafish danio rerio. Zebrafish protein antibody scbt santa cruz biotechnology. To address this, we examined the function of the ir during zebrafish development. Zebrafish akt2 is essential for survival, growth, bone. The exact definition of a genetically modified organism and what constitutes genetic engineering varies, with the most common being an organism altered in a way that does not occur naturally by mating andor natural recombination. Our anti akt antibodies and pi3kinase pathway reagents help monitor levels of akt and its phosphorylation at position s473, t308, as well as akt antibody isoforms akt2 and akt3. Antibodies research products elisa kits antibodies.
Akt antibodies molecular mechanisms of cancer sigmaaldrich. A 28h wholemount zebrafish embryo labeled with an antibody which recognizes the transcription factor, pax6 brown label showing a lateral view with anterior to the left. It is aimed to help labome visitors find the most suited akt antibody. As the vast majority of commercial antibodies are raised against human proteins, we became interested in taking a look at which commercial antibodies are being used by the zebrafish research community. Labome lists 954119 antibody products against 88487 genes, including 19044 human, 15677 mouse, and 14548 rat genes. Antibody detects endogenous levels of total akt1, akt2 and akt3 proteins. Anti fish antibody products listed below are anti fish antibodies from multiple suppliers. Although nodrip2 axis conservatively existed in the teleost, the function of rip2 was only reported in zebrafish, goldfish, and rainbow trout in vitro.
Role of insulin receptor signaling in zebrafish embryogenesis. Our product portfolio covers all common host organisms and reactivity types. Very little is known about the role and mechanisms of piscine nodrip2 axis in vivo. The 13amino acid protein is a member of the sh3pxd2 family. Select zebrafish protein antibodies from monoclonal antibodies listed below. I have tried antibodies against ki67 nclki67p and 550609 and pcna. Fish is a reported alias name for the human gene sh3pxd2a, or sh3 and px domains 2a. All antibodies listed on this page were generated by researchers at the institute of neuroscience, university of oregon liu et al. The prices we charge for products and services are to offset some of the actual costs we incur. Cell signaling technologys akt antibody is a rabbit polyclonal antibody. Abcam antibodies and reagents supplier, find any antibody. Downstream of fgfrpi3kakt signaling, sox9a and atoh1a bind. Ligand binding to vegfr2 activates both the mapkerk and the pi3kakt signaling pathways, 166,169,170 and functional conservation of both has also been demonstrated in the zebrafish model. Recent advances in akt signaling have focused on understanding cellular processes and identifying cellular substrates.